Four simple chemometric assisted UV-Spectrophotometric methods, classical least square (CLS), inverse least square (ILS), principal component regression (PCR) and partial least squares (PLS) were developed for simultaneous estimation of Cefixime Trihydrate (CEF) and Linezolid (LINE) in their combined dosage tablet formulation without any chemical separation and any graphical treatment of the overlapping spectra of two drugs. The UV absorption spectra of the studied drugs, in the range of 235–295 nm, showed a considerable degree of spectral overlapping. Beer’s law was obeyed for both drugs in the general concentration ranges of 2–6 µg/ml and 6–18 µg/ml for CEF and LINE, respectively. Eighteen mixed solutions were prepared for the chemometric calibration as training set and eight mixed solutions were prepared as validation set. The absorbance data matrix was obtained by measuring the absorbance at twenty one wavelength points, from 235 to 295 nm with the interval of 3 nm (Δ=3 nm). The developed calibrations were successfully tested for laboratory mixtures as well as commercial tablet formulation for their CEF and LINE concentration.
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